2022 Spring Round of The Emerald Test™

Enrollment for the 2022 Spring Round opens February 7, 2022

General Instructions for Participants

Thank you for your interest in the 2022 Spring Emerald Test™, the Inter-Laboratory Comparison Proficiency Test (ILC/PT) program for cannabis and hemp testing facilities. This section contains an overview of details relating to enrollment, the proficiency testing process, instructions for result submission, and Emerald Badge™ award criteria.

Please read this document thoroughly. All questions should be addressed to:

Ken Groggel, Director of PT Programs
(877) 567-3598 ext. 709
ken.g@emeraldscientific.com

Once the Open enrollment period for the Spring Round of The Emerald Test™ opens on February 7, simply scroll down and purchase individual PTs from the options below, or contact us at sales@emeraldscientific.com, or (877) 567-3598 ext. 1. If you’re not sure which PTs to purchase, your Emerald Scientific Technical Sales Specialist is always available to answer questions and help.

Once Open Enrollment begins on August 11, the fees for participating in The Emerald Test™ may be seen by clicking on the “Enroll Now” button next to an individual PT and following the link under each series. It is very important that the testing facility name, analyst email, and physical address are included when purchasing a PT to avoid shipping and communication problems.

EventDate
Open Enrollment Period BeginsFriday, February 7
Open Enrollment Period ClosesFriday, February 18
Samples ship for International labs:Monday, March 14
U.S. / Canada Samples ship:Wednesday, March 16
Results reporting for all PTs closes:Friday, April 8
Initial Reports released / Appeals open:Wednesday, April 20
Appeals close:Friday, April 29
Advisory Panel review:Wednesday, May 11
Emerald Badges™ awarded: Friday, May 27
ILC report released:Friday, June 3

Inter-laboratory comparison data is presented in anonymized format according to a randomly assigned analyst number. The Emerald Test™ will keep all laboratory, analyst information, and results confidential. Participant information will not be released by The Emerald Test™ without the prior knowledge and consent of the participating laboratory. Should a regulatory agency or other entity need to receive the results, permission is required from the participant authorizing The Emerald Test™ to release test results.

The dates that samples will be shipped and the date that results are due are both listed above and in the instructions included with the PT samples. Prior to opening the sample, please review the sample preparation instructions.  To accommodate the PT, please follow the manufacturers’ sample preparation notes that go with each part number.

If samples shipped within the U.S. and Canada are not received within 48 hours of the ship date, notify the PT manufacturer. Failure to do so after 5 working days from the ship date may result in a forfeited test. International shipments may take longer.

Be sure to thoroughly inspect all samples and review all documents upon receipt and store under the conditions recommended by the ISO accredited PT manufacturer. Damaged samples should be reported immediately to the PT Manufacturer and Ken Groggel. Failure to do so within 1 week of the ship date may result in a forfeited test.

Each participant is responsible for selecting an analysis method appropriate for the samples they are testing. Some series will request that the participant list their analysis method in a general fashion (e.g. LC, GC, PCR, LC-MS/MS, etc.). The method provided will be entered with their results in the appropriate boxes on the results entry form. This anonymized data is used to monitor developments or changes in methods within the industry and to assist in future test development. Contact the ISO accredited PT manufacturer if you have any questions or concerns regarding the reporting process.

Enrollment in The Emerald Test™ is a participant’s implied assertion that the laboratory name provided when purchasing The Emerald Test™ is the laboratory that performs the sample analysis. Any attempt to circumvent this agreement will result in a forfeited test.

Carefully follow the ISO accredited PT manufacturer’s instructions when submitting your results. Sample results must be submitted by the due date. Late results will not be accepted, will not be added to the database, and will be treated as unreported. Typographical errors will not be considered for an appeal.

If you DO NOT want to report results for a particular analyte within the PT, leave it blank.  Any number or image within an analyte cell will be viewed as a testing outcome and scored as such.

Accurate data reporting is part of the analysis process, thus all participants are responsible for review of their own reported results to ensure accuracy. Be sure to review the documents included with the PT samples for data entry instructions. It is recommended that a second person review your reported results for accuracy. Contact the ISO accredited PT manufacturer if you have any questions or concerns regarding the reporting process. Incorrectly reported results will not be changed after the Series closing date. There are no exceptions.

There are 2 separate internet portals for labs to enter their PT results, one for each manufacturer.  Review and follow the directions provided by the ISO accredited PT manufacturer, which will include the information for the correct portal to use to enter your results. Participant access to reports is limited to their enrolled tests. 

Contact the PT manufacturer of your PT(s) directly with any questions.

Initial reports will be available from the PT manufacturer no later than April 20, 2022. If the facility name, analyst email, and physical address for the lab performing the PT analysis are not correct when the sample is purchased, your results may be delayed or voided. It is recommended that you review your report carefully and direct any questions to the PT manufacturer before entering the data.

Be aware of the requirements and limitations when considering an appeal to ensure your request is based on reasonable testing parameters. All appeals must be received by the PT manufacturer no later than April 29, 2022 to be considered. Appeal requests must include:

  • Laboratory name
  • PT Series/test number
  • Appeal description

All appeals must be submitted to the ISO accredited manufacturer. Typographical errors will not be considered for an appeal. Appeals submitted after April 29 will not be considered.

An Emerald Badge™ will be awarded to participating labs that meet the criteria established by the ISO accredited PT manufacturer with input from The Emerald Test™ Advisory Panel. Labs receiving a Badge are recognized for their capabilities through inter-laboratory comparison with their peers for a given test. Be advised that a minimum of 8 participants are required to complete the ILC/PT analysis. The ILC/PT promotes transparency and enhances consumer confidence in the safety and the science which independent testing facilities contribute to the cannabis and hemp industries.

Once again, thank you for participating in The Emerald Test™! Should you have any questions or suggestions for improving the experience and value to your business, please let us know.

2021 FALL PTs

While you wait for the Open Enrollment Period for our 2022 Spring Round, learn more about the PTs offered in our 2021 Fall Round below.

Take advantage of this time to run some proficiency tests before participating in 2022 Spring Round of The Emerald Test™. We’ve got you covered with the best cannabis proficiency tests on the market, all just a click away!

Shop specific PTs including metals, microbiology, pesticides, pH, potency, residual solvents, terpenes, water activity tests, and more!

The 2021 Fall Round of The Emerald Test™ offered a broad range of PTs to meet a laboratory’s internal SOP, regulatory compliance, and ISO/IEC accreditation needs. The details of each, as well as the ILC Reports, are available for your review below.

PT Manufacturer A

7 part Potency in Solution
Cannabinoid analysis
Components: Acetonitrile matrix (1 mL sample, 10 – 1000 µg/mL)

  • Δ8-Tetrahydrocannabinol (THC) CAS# 5957-57-5
  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabigerol (CBG) CAS# 25654-31-3
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test and CRM, may be purchased in addition to this test.

7 part Potency in Hemp Oil
Cannabinoid analysis
Components: Hemp Oil matrix (1 mL sample, 10 – 1000 µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7
  • Cannabigerol (CBG) CAS# 25654-31-3
  • Δ8-Tetrahydrocannabinol (THC) CAS# 5957-57-5

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

5 part Potency in Hemp Bud
Cannabinoid analysis
Components: Hemp Bud matrix (1g sample, 10 – 80,000 µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

7 part Potency in Gummy
Cannabinoid analysis
Components: Gummy matrix  (1 piece sample, 10 – 1,000 µg/g)
Contains 1 – 7 possible analytes:

  • Δ8-Tetrahydrocannabinol (THC) CAS# 5957-57-5
  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabigerol (CBG) CAS# 25654-31-3
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

5 part Potency in Beverage
Cannabinoid analysis
Components: Infused water matrix (1 mL sample, 10 – 1,000 µg/mL)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test and CRM, may be purchased in addition to this test.

5 part Potency in Hard Candy
Cannabinoid analysis
Components: Hard candy matrix (1 piece sample, 10 – 1,000 µg/g)
Contains 1 – 5 possible analytes:

  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test. 

5 part Potency in Chocolate
Cannabinoid analysis
Components: Chocolate syrup matrix  (1g sample, 10 – 1,000 µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

23 part Residual Solvents in Hemp Oil
Solvent analysis
Components: Hemp oil matrix; At least 60% will be present (2.0 mL sample, 10 – 1,000 µg/g)

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

Analyte / CAS #Analyte / CAS #
Acetone 67-64-1Acetonitrile 75-05-8
Benzene 71-43-2Chloroform 67-66-3
1,2-Dichloroethane 107-06-2Diethyl ether (Ethyl ether) 60-29-7
Ethanol 64-17-5Ethyl acetate 141-78-6
Ethylene oxide 75-21-8Heptane 142-82-5
Hexane 110-54-3Isopropyl Alcohol- Isopropanol (2-Propanol) 67-63-0
Methanol 67-56-1Methylene chloride 75-09-2
Pentane 109-66-0Toluene 108-88-3
Trichloroethene 79-01-6Butane 106-97-8
Iso-butane 75-28-5Propane 74-98-6
m-Xylene 108-38-3o-Xylene 95-47-6
p-Xylene 106-42-3

4 part Heavy Metals in Hemp Oil
Heavy Metals analysis
Components: Hemp oil matrix (0.5 g sample, varied µg/g)

  • Arsenic CAS# 7440-38-2
  • Cadmium CAS# 7440-43-9
  • Lead CAS# 7439-92-1
  • Mercury CAS# 7939-97-6

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

5 part Heavy Metals in Hemp Bud
Heavy Metals analysis
Components: Hemp bud matrix (Available in 0.2, 0.5 or 1g sample sizes, varied µg/g)
  • Arsenic CAS# 7440-38-2
  • Cadmium CAS# 7440-43-9
  • Lead CAS# 7439-92-1
  • Mercury CAS# 7939-97-6
  • Chromium CAS# 7789-02-8
A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

10 part Terpenes in Hemp Bud
Terpene analysis
Components: Hemp bud matrix (1 g sample, range 10 – 5,000 µg/g)

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

Analyte / CAS #Analyte / CAS #
alpha-Pinene 80-56-8R)-(+)-Limonene 5989-27-5
1S)-(-)-beta-Pinene 18172-67-3Linalool 78-70-6
beta-Myrcene 123-35-3beta–Caryophyllene 87-44-5
Caryophyllene oxide 1139-30-6alpha–Humulene 6753-98-6
alpha-Terpinolene 586-62-9(-)-alpha–Bisabolol 23089-26-1

7 part Water Activity in Hemp Bud
Humidity analysis
Components: Hemp bud matrix (0.5 g sample, range 0.08 – 1 µg/g)

  • Potassium sulfate CAS# 7778-80-5
  • Potassium nitrate CAS# 7757-79-1
  • Potassium chloride CAS# 7447-40-7
  • Potassium Hydroxide CAS# 01310-58-3
  • Ammonium sulfate CAS# 7783-20-2
  • Sodium chloride CAS# 7647-14-5
  • Sodium nitrite CAS# 7632-00-0

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

Vitamin E Acetate in Hemp Oil
Contaminate analysis
Components: Hemp oil matrix (1 mL sample, 10 – 1,000 µg/mL)

(+/-)-alpha-Tocopherol acetate  CAS# 7695-91-2

A Practice Test, CRM, and Blank Matrix may be purchased in addition to this test.

PT Manufacturer B

Pesticide Screening in Hemp
Pesticide analysis
(Available in 0.5, 1, or 1.5 g sample sizes, varied µg/g)

The sample will be evaluated quantitatively for the following residues in hemp. At least 25% will be present at 0.30 to 3.00 ppm. Each PT is supplied with a blank matrix and 3 mL (2 x 1.5 mL) of spiking solution to allow for duplicate analysis if needed. Sample must be analyzed in its entirety to assure intra sample homogeneity. 

A Practice Test may be purchased in addition to this test.

Analyte / CAS #Analyte / CAS #Analyte / CAS #
2-Chloroethyl phosphonic acid 16672-87-0Dimethomorph 110488-70-5Myclobutanil 88671-89-0
Abamectin (B1B / B1A) 71751-41-2Dinotefuran 165252-70-0Naled 300-76-5
Acephate 30560-19-1Diuron 330-54-1Novaluron 116714-46-6
Acequinocyl 57960-19-7Dodemorph 1593-77-7Oxamyl 23135-22-0
Acetamiprid 135410-20-7Endosulfan sulfate 1031-07-8Paclobutrazol 767738-62-0
Aldicarb 116-06-3Endosulfan-alpha 959-98-8Pentachloronitrobenzene 82-68-8
Allethrin 584-79-2Endosulfan-beta 33213-65-9Permethrin 52645-53-1
Ancymidol 12771-68-5Ethoprophos 13194-48-4Phenothrin 26002-80-2
Atrazine 1912-24-9Etofenprox 80844-07-1Phosmet 732-11-6
Avermectin 71751-41-2Etoxazole 153233-91-1Piperonyl butoxide 51-03-6
Azadirachtin 11141-17-6Etridiazol 2593-15-9Pirimicarb 23103-98-2
Azoxystrobin 131860-33-8Fenhexamid 126833-17-8Prallethrin 23031-36-9
Benzovindiflupyr 1072957-71-1Fenoxycarb 72490-01-8Propiconazole 60207-90-1
Bifenazate 149877-41-8Fenpyroximate 111812-58-9 & 134098-61-6Propoxur 114-26-1
Bifenthrin 82657-04-3Fensulfothion 115-90-2Pyraclostrobin 175103-18-0
Boscalid 188425-85-6Fenthion 55-38-9Pyrethrins 8003-34-7
Buprofezin 69327-76-0Fenvalerate 51630-58-1Pyridaben 96489-71-3
Captan 133-06-2Fipronil 120068-37-3Pyriproxyfen 95737-68-1
Carbaryl 63-25-2Flonicamid 158062-67-0Resmethrin 10453-86-8
Carbofuran 1563-66-2Fludioxonil 131341-86-1Spinetoram 187166-15-0 & 187166-40-1
Chlorantraniliprole 500008-45-7Fluopyram 658066-35-4
Spinosad 168316-95-8
Chlordane 57-74-9Flurprimidol 56425-91-3Spirodiclofen 148477-71-8
Chlormequat chloride 999-81-5Hexythiazox 78587-05-0Spiromesifen 283594-90-1
Chlorphenapyr 122453-73-0Imazalil 35554-44-0Spirotetramat 203313-25-1
Chlorpyrifos 2921-88-2Imidacloprid 138261-41-3Spiroxamine 118134-30-8
Clofentezine 74115-24-5Iprodione 36734-19-7Tebuconazole 107534-96-3
Clothianidin 210880-92-5Kinoprene 42588-37-4
Tebufenozide 112410-23-8
Coumaphos 56-72-4Kresoxim-methyl 143390-89-0Teflubenzuron 83121-18-0
Cyantraniliprole 736994-63-1Lambda-Cyhalothrin 91465-08-6Tetrachlorvinphos 22248-79-9
Cyfluthrin 68359-37-5 & 1820573-27-0Malathion 121-75-5Tetramethrin 7696-12-0
Cypermethrin 52315-07-8Metalaxyl 57837-19-1Thiabendazole 148-79-8
Cyprodinil 121552-61-2Methiocarb 2032-65-7Thiacloprid 111988-49-9
Daminozide 1596-84-5Methomyl 16752-77-5
Thiamethoxam 153719-23-4
Deltamethrin 52918-63-5Methoprene 40596-69-8Thiophanate-methyl 23564-05-8
Diazinon 333-41-5Methyl parathion 298-00-0Trifloxystrobin 141517-21-7
Dichlorvos 62-73-7Mevinphos 7786-34-7
Dimethoate 60-51-5MGK-264 113-48-4

Microbial Panel 1 in Hemp
(1 g hemp sample)

A 4 sample panel for quantitative determination of APC, Total Coliform, E.coli, Enterobacteriaceae and Total Yeast/Mold. Each Supplied in a 1 g hemp matrix, lyophilized. Supplied with 1 vial of Hydration fluid.

A Practice Test and CRM may be purchased in addition to this test.

Microbial Panel 2 – Salmonella in Hemp
(1 g hemp sample)

A 5 sample panel for qualitative identification of Salmonella (spp) supplied in a hemp matrix, lyophilized. Supplied with 1 vial of sterile deionized water. 3 series available per study. At least 2 samples of each 5 sample series test positive for salmonella. The negative samples may contain non-salmonella, non-pathogenic species.  Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 3 – STEC in Hemp
(1 g hemp sample)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) supplied in a hemp matrix, lyophilized.  Supplied with 1 vial of sterile deionized water.  At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli.  Evaluated for total STEC and/or specific serotype.  Applicable to cultural or molecular techniques. 
Note: domestic shipping only.
A practice test and positive control may be purchased in addition to this test.

Microbial Panel 4 – Aspergillus Molds in Hemp
(1 g hemp sample)

A 10 sample panel for qualitative identification of mold in a hemp matrix, lyophilized. Supplied with 2 vials of sterile deionized water. At least 4 samples will contain a pathogenic Aspergillus (spp) for determination (A. fumagatis, A. flavus, A. niger, A. terreus). Applicable to cultural or molecular techniques. 

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 5 – Salmonella in Chocolate
(1 piece samples)

A 5 sample panel for qualitative identification of salmonella in a chocolate matrix.  At least 2 samples of each 5 sample series test positive for salmonella.  Supplied with 1 vial of sterile deionized water. Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 6 – STEC in Chocolate
(1 piece samples)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) in a chocolate matrix.  At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli.  Supplied with 1 vial of sterile deionized water.  Evaluated for total STEC and/or specific serotype. Applicable to cultural or molecular techniques. 
Note: domestic shipping only.
A practice test and positive control may be purchased in addition to this test.

Microbial Panel 7 – Salmonella in Hemp Oil
(1.2 g hemp oil sample)

A 5 sample panel for qualitative identification of salmonella (spp) in hemp oil.  At least 2 samples of each 5 sample series test positive for salmonella.  Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 8 – STEC in Hemp Oil
(1.2 g hemp oil sample)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) in hemp oil. At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli. Evaluated for total STEC and/or specific serotype.  Applicable to cultural or molecular techniques.
Note: domestic shipping only.
A practice test and positive control may be purchased in addition to this test.

Microbial Panel 9 – Aspergillus Molds in Hemp Oil
(1.2 g hemp oil sample)

A 10 sample panel for qualitative identification of aspergillus molds in hemp oil.  At least 4 samples will contain a pathogenic Aspergillus (spp) for determination (A. fumagatis, A. flavus, A. brasilliensis(Niger), A. terreus).  Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Mycotoxins in Hemp
(1 g hemp sample)

A sample set for quantitative determination of Ochratoxin A and Total Aflatoxin (B1, B2, G1, and G2). The sample will contain Ochratoxin A at 5 – 50 ppb, and 2 of the 4 possible aflatoxins at  5 – 50 ppb each. Sample must be analyzed in its entirety to assure intra sample homogeneity. PT sample set includes an acetonitrile based spiking solution and blank matrix.  Enough mass and volume is supplied for duplicate assay if needed.

  • Ochratoxin A  CAS# 303-47-9
  • Aflatoxin B1  CAS# 1162-65-8
  • Aflatoxin B2  CAS# 7220-81-7
  • Aflatoxin G1  CAS# 1165-39-5
  • Aflatoxin G2  CAS# 7241-98-7

A practice test and positive control may be purchased in addition to this test.

Foreign Materials in Hemp
(500 mg in hemp)

A 5 sample panel for semi-quantitative determination of foreign materials in hemp matrix. At least 2 of 5 samples will contain foreign materials at levels above 5% by mass. Each vial will contain 500 mg of materials for analysis. Participants will report results for each sample of the panel.

A practice test and positive control may be purchased in addition to this test.

Listeria in Hemp

A 5 sample panel for qualitative identification of Listeria monocytogenes in a hemp matrix.  At least 2 of the 5 samples in the panel will be positive for listeria monocytogenes.  Applicable to cultural and/or molecular techniques.

A practice test and positive control may be purchased in addition to this test.