FALL 2020 TEST

Fall enrollment is closed. Spring enrollment dates will soon be announced.

General Instructions for Participants

Thank you for your interest in the Fall 2020 Emerald Test™, the Inter-Laboratory Comparison and Proficiency Test (ILC/PT) for cannabis and hemp testing facilities. This section contains an overview of details relating to enrollment, the proficiency testing process, instructions for result submission and Emerald Test™ award criteria.

Please read this document thoroughly. All questions should be addressed to:

Ken Groggel, PT Director
(877) 567-3598 ext. 709
ken.g@emeraldscientific.com

Enrollment in the Emerald Test™ is done by purchasing individual PTs from the menu below, by contacting sales@emeraldscientific.com or (877) 567-3598 ext. 1. Your Emerald Scientific Technical Sales Specialist is also there to help. Fees for Emerald Test™ participation are found by clicking on the “Buy Now” button and following the link under each series. It is very important that the testing facility name, analyst email and physical address are included when purchasing a PT to avoid shipping and communication problems.

EventDate
EnrollmentAugust 19 – September 2
Samples ship for International labs:September 18
US/Canada Samples ship for PT Manufacturer A:

  • Cannabinoid Potency

  • Residual Solvents

  • Heavy Metals

  • Terpenes

  • Water Activity

  • Vitamin E Acetate

September 23
US/Canada Samples ship for PT Manufacturer B:

  • Pesticides

  • Microbial Panel

  • Microbial Panels-Salmonella

  • Microbial Panels-STEC

  • Microbial Panels-Aspergillus Mold

  • Foreign Material

September 23
Results reporting for all PTs closes:October 9
Initial Reports released/Appeals open:October 23
Appeals close:November 6
Advisory Panel review:November 18
Emerald Badges awarded/ILC report released:December 11

Inter-Laboratory comparison data is presented in anonymized format according to a randomly assigned analyst number. The Emerald Test™ will keep all laboratory, analyst information and results confidential. Participant information will not be released by the Emerald Test™ without the prior knowledge and consent of the participating laboratory. Should a regulatory agency or other entity need to receive the results, permission is required from the participant authorizing the Emerald Test™ to release test results.

Sample ship date and results due date are listed above and in the instructions included with the PT samples.

If samples are not received within 48 hours of the ship date, notify the PT manufacturer. Failure to do so after 5 days from the ship date may result in a forfeited test. International shipments may take longer.

Be sure to thoroughly inspect all samples and review all documents upon receipt and store under the conditions recommended by the ISO accredited PT manufacturer. Damaged samples should be reported immediately to the PT Manufacturer and Ken. Failure to do so within 1 week of the ship date may result in a forfeited test.

It is the responsibility of the participant to choose an analysis method appropriate for the samples they are testing. Some series will request that the participant list their analysis method in a general fashion (e.g. LC, GC, PCR, LC-MS/MS etc.). These will be entered with their results in the appropriate boxes on the results entry form. This anonymized data is used to monitor developments or changes in technique in the industry and to assist in future test development. Contact the ISO accredited PT manufacturer if you have any questions or concerns regarding the reporting process.

Enrollment in the Emerald Test™ is a participants’ implied assertion that the laboratory name provided when purchasing the Emerald Test™ is the laboratory that performs the sample analysis.

Carefully follow the ISO accredited PT manufacturers’ instructions when submitting your results. Sample results must be submitted by the due date. Late results will not be accepted. They will not be added to the database and will be treated as unreported. Typographical errors will not be considered for an appeal.

Accurate data reporting is part of the analysis process, thus all participants are responsible for review of their own reported results to ensure accuracy. Be sure to review the documents included with the PT samples for data entry instructions. It is recommended that a second person review your reported results for accuracy. Contact the ISO accredited PT manufacturer if you have any questions or concerns regarding the reporting process. Incorrectly reported results will not be changed after the Series closing date. There are no exceptions.

There are 2 separate internet portals for labs to enter their PT results, one for each manufacturer. Our intention is to help participating labs to easily identify the ISO accredited PT manufacturer portal that matches a given test. Review and follow the directions provided by the ISO accredited PT manufacturer. Contact the manufacturer directly with any questions. Participant access to reports is limited to their enrolled tests.

Initial reports will be available from the PT manufacturer no later than October 23. If the facility name, analyst email and physical address for the lab performing the PT analysis is not correct when the sample is purchased, your results may be delayed or voided. It is recommended that you review your report carefully and direct any questions to the PT manufacturer before entering the data.

Be aware of the requirements and limitations when considering an appeal to ensure your request is based on reasonable testing parameters. All appeals must be received by the PT manufacturer no later than November 6 to be considered. Appeal requests should include:

Laboratory name
Series/test number
Appeal description

All appeals must be submitted to the ISO accredited manufacturer. Typographical errors will not be considered for an appeal. Appeals submitted after November 6 will not be considered.

An Emerald Badge™ will be awarded to participating labs that meet the criteria established by the ISO accredited PT manufacturer with input from the Emerald Test™ Advisory Panel. Labs receiving a Badge are recognized for their capabilities through Inter-Laboratory comparison with their peers for a given test. Be advised that a minimum of 8 participants are required to complete the ILC/PT analysis. The ILC/PT promotes transparency and enhances consumer confidence in the safety and science independent testing facilities contribute to the cannabis and hemp industry.

Once again, thank you for participating in the Emerald Test™. Should you have any questions or suggestions for improving the experience and value to your business, please let me know.

THE TESTS

The Fall 2020 Emerald Test™ offers a broad range of PTs to meet a laboratory’s internal SOP, regulatory compliance and ISO/IEC accreditation needs.

PT Manufacturer A

7 part Potency in Solution –  cannabinoid analysis. Components: Acetonitrile matrix. (1 mL sample, 10 – 1000µg/mL)

  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7
  • Cannabigerol (CBG) CAS# 25654-31-3
  • Δ8-Tetrahydrocannabinol (THC) CAS# 5957-57-5

A Practice Test and CRM may be purchased in addition to this test.

5 part Potency in Hemp Oil – cannabinoid analysis. Components: Hemp Oil matrix. (1 mL sample, 10 – 1000µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

5 part Potency in Hemp Bud – cannabinoid analysis. Components: Hemp Bud matrix. (1g sample, 10 – 80,000µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

5 part Potency in Gummy – cannabinoid analysis. Components: Gummy matrix. (1 piece sample, 10 – 1,000µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

5 part Potency in Beverage – cannabinoid analysis. Components: Infused water matrix. (1 mL sample, 10 – 1000µg/mL)

  • Δ9-Tetrahydrocannabinol (THC) CAS #1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test and CRM may be purchased in addition to this test.

5 part Potency in Hard Candy – cannabinoid analysis. Components: Hard candy matrix. (1 piece sample, 10-1000µg/g)

  • Δ9-Tetrahydrocannabinol (THC) CAS# 1972-08-3
  • Δ9-tetrahydrocannabinolic Acid (THCA) CAS# 23978-85-0
  • Cannabidiol (CBD) CAS# 13956-29-1
  • Cannabidiolic Acid (CBDA) CAS# 1244-58-2
  • Cannabinol (CBN) CAS# 521-35-7

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

CBD in Topical Cream  – cannabinoid analysis. Components: Cream matrix w/beeswax base. (1g sample, range 10-1000 µg/g)

  • Cannabidiol (CBD) CAS# 13956-29-1

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test

22 part Residual Solvents in Hemp Oil
Solvent analysis. Components: Hemp oil matrix. At least 60% will be present. (2.0 mL sample, 10 – 1000µg/g)

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

Analyte / CAS #Analyte / CAS #
Acetone 67-64-1Acetonitrile 75-05-8
Benzene 71-43-2Chloroform 67-66-3
1,2-Dichloroethane 107-06-2Diethyl ether (Ethyl ether) 60-29-7
Ethanol 64-17-5Ethyl acetate 141-78-6
Ethylene oxide 75-21-8Heptane 142-82-5
Hexane 110-54-3Isopropyl Alcohol- Isopropanol (2-Propanol) 67-63-0
Methanol 67-56-1Methylene chloride 75-09-2
Pentane 109-66-0Toluene 108-88-3
Trichloroethene 79-01-6Butane 106-97-8
m-Xylene 108-38-3Propane 74-98-6
o-Xylene 95-47-6p-Xylene 106-42-3

4 part Heavy Metals in Hemp Oil

Heavy Metals analysis. Components: Hemp oil matrix. (0.6g sample, varied µg/g)

  1. Arsenic CAS# 7440-38-2
  2. Cadmium CAS# 7440-43-9
  3. Lead CAS# 7439-92-1
  4. Mercury CAS# 7939-97-6

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

5 part Heavy Metals in Hemp Bud

Heavy Metals analysis. Components: Hemp bud matrix. (1g sample, varied µg/g)

  1. Arsenic CAS# 7440-38-2
  2. Cadmium CAS# 7440-43-9
  3. Lead CAS# 7439-92-1
  4. Mercury CAS# 7939-97-6
  5. Chromium CAS# 7789-02-8

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

10 part Terpenes in Hemp Bud – Terpene analysis. Components: Hemp bud matrix.

(1g sample, range 10-5000µg/g).

 

Analyte / CAS #Analyte / CAS #
alpha-Pinene 80-56-8R)-(+)-Limonene 5989-27-5
1S)-(-)-beta-Pinene 18172-67-3Linalool 78-70-6
beta-Myrcene 123-35-3beta–Caryophyllene 87-44-5
Caryophyllene oxide 1139-30-6alpha–Humulene 6753-98-6
alpha-Terpinolene 586-62-9(-)-Alpha–Bisabolol 23089-26-1

7 part Water Activity on hemp bud – Humidity analysis.  Components: Hemp bud matrix. (0.5g sample, range 0.08-1.0 aW)

  • Potassium sulfate CAS# 7778-80-5
  • Potassium nitrate CAS# 7757-79-1
  • Potassium chloride CAS# 7447-40-7
  • Potassium Hydroxide CAS# 01310-58-3
  • Ammonium sulfate CAS# 7783-20-2
  • Sodium chloride CAS# 7647-14-5
  • Sodium nitrite CAS# 7632-00-0

A Practice Test, CRM and Blank Matrix may be purchased in addition to this test.

Vitamin E Acetate – Contaminate analysis.  Components: Methanol matrix. (1mL sample, 1-1000µg/mL)

(+/-)-alpha-Tocopherol acetate  CAS# 7695-91-2

A Practice Test and CRM may be purchased in addition to this test.

PT Manufacturer B

Pesticide Screening in Hemp – Pesticide analysis. (Available in 0.5, 1 or 1.5g sample sizes, varied µg/g)

The sample will be evaluated quantitatively for the following residues in hemp.  At least 25% will be present at 0.1 to 400ppm.  Range is based upon a multiple of BCC action levels.  Each PT is supplied with a blank matrix and 3mL of spiking solution to allow for duplicate analysis if needed.

A Practice Test may be purchased in addition to this test.

Analyte / CAS #Analyte / CAS #Analyte / CAS #
Aldicarb 116-06-3Carbofuran 1563-66-2 Chlordane 57-74-9
Chlorfenapyr 122453-73-0Chlorpyrifos 2921-88-2 Coumaphos 56-72-4
Daminozide 1596-84-5 DDVP (Dichlorvos) 62-73-7 Dimethoate 60-51-5
Ethoprop(hos) 13194-48-4 Etofenprox 80844-07-1 Fenoxycarb 72490-01-8
Fipronil 120068-37-3Imazalil 35554-44-0Methiocarb 2032-65-7
Methyl parathion 298-00-0Mevinphos 7786-34-7 Paclobutrazol 76738-62-0
Propoxur 114-26-1 Spiroxamine 118134-30-8 Thiacloprid 111988-49-9
Abamectin 71751-41-2 Acephate 30560-19-1 Acequinocyl 57960-19-7
Acetamiprid 135410-20-7 Azoxystrobin 131860-33-8 Bifenazate 149877-41-8
Bifenthrin 82657-04-3 Boscalid 188425-85-6 Captan 133-06-2
Carbaryl 63-25-2 Chlorantraniliprole 500008-45-7 Clofentezine 74115-24-5
Cyfluthrin 68359-37-5 Cypermethrin 52315-07-8 Diazinon 333-41-5
Dimethomorph 110488-70-5 Etoxazole 153233-91-1 Fenhexamid 126833-17-8
Fenpyroximate 134098-61-6 Flonicamid 158062-67-0 Fludioxonil 131341-86-1
Hexythiazox 78587-05-0 Imidacloprid 138261-41-3 Kresoxim-methyl 143390-89-0
Malathion 121-75-5 Metalaxyl 57837-19-1 Methomyl 16752-77-5
Myclobutanil 88671-89-0 Naled 300-76-5 Oxamyl 23135-22-0
Pentachloronitrobenzene 82-68-8Permethrin 52645-53-1 Phosmet 732-11-6
Piperonylbutoxide 51-03-6 Prallethrin 23031-36-9 Propiconazole 60207-90-1
Pyrethrins 8003-34-7 Pyridaben 96489-71-3 Spinetoram 187166-40-1
Spinosad 168316-95-8Spiromesifen 283594-90-1 Spirotetramat 203313-25-1
Tebuconazole 107534-96-3 Thiamethoxam 153719-23-4Trifloxystrobin 141517-21-7

Microbial Panel 1 in Hemp
(1g hemp sample)

A 4 sample panel for quantitative determination of APC, Total Coliform, E.coli, Enterobacteriaceae and Total Yeast/Mold. Each Supplied in a 1g hemp matrix, lyophilized. Supplied with 1 vial of Hydration fluid.

A Practice Test and CRM may be purchased in addition to this test.

Microbial Panel 2 – Salmonella in Hemp
(1g hemp sample)

A 5 sample panel for qualitative identification of Salmonella(spp) supplied in a hemp matrix, lyophilized. Supplied with 1 vial of sterile deionized water. At least 2 samples of each 5 sample series test positive for salmonella. The negative samples may contain non-salmonella, non-pathogenic species.  Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 3 – STEC in Hemp
(1g hemp sample)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) supplied in a hemp matrix, lyophilized.  Supplied with 1 vial of sterile deionized water.  At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli.  Evaluated for total STEC and/or specific serotype.  Applicable to cultural or molecular techniques.  Note: domestic shipping only.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 4 – Aspergillus Molds in Hemp
(1g hemp sample)

A 10 sample panel for qualitative identification of mold in a hemp matrix, lyophilized. Supplied with 2 vials of sterile deionized water. At least 4 samples will contain a pathogenic Aspergillus(spp) for determination (A. fumagatis, A. flavus, A. niger, A. terreus). Applicable to cultural or molecular techniques. 

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 5 – Salmonella in Chocolate
(1 piece samples)

A 5 sample panel for qualitative identification of salmonella in a chocolate matrix.  At least 2 samples of each 5 sample series test positive for salmonella.  Supplied with 1 vial of sterile deionized water. Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 6 – STEC in Chocolate
(1 piece samples)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) in a chocolate matrix.  At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli.  Supplied with 1 vial of sterile deionized water.  Evaluated for total STEC and/or specific serotype. Applicable to cultural or molecular techniques.  Note: domestic shipping only.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 7 – Salmonella in Hemp Oil
(1.2g hemp oil sample)

A 5 sample panel for qualitative identification of salmonella(spp) in hemp oil.  At least 2 samples of each 5 sample series test positive for salmonella.  Applicable to cultural or molecular techniques.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 8 – STEC in Hemp Oil
(1.2g hemp oil sample)

A 5 sample panel for qualitative identification of STEC (O26, O45, O103, O111, O121, O145) in hemp oil. At least 2 samples of each 5 sample series will test positive for shiga-toxin expressing E.coli. Evaluated for total STEC and/or specific serotype.  Applicable to cultural or molecular techniques.  Note: domestic shipping only.

A practice test and positive control may be purchased in addition to this test.

Microbial Panel 9 – Aspergillus Molds in Hemp Oil
(1.2g hemp oil sample)

A 10 sample panel for qualitative identification of aspergillus molds in hemp oil.  At least 4 samples will contain a pathogenic Aspergillus(spp) for determination (A. fumagatis, A. flavus, A. niger, A. terreus).  Applicable to cultural or molecular techniques. 

A practice test and positive control may be purchased in addition to this test.

Mycotoxins in Hemp
(1g hemp sample)

A sample set for quantitative determination of Ochratoxin A, and Total Aflatoxin (B1, B2, G1 and G2). The sample will contain Ochratoxin A at 5-50ppb, and 2 of the 4 possible aflatoxins at          5-50ppb each. PT sample set includes an acetonitrile based spiking solution and blank matrix.  Enough mass and volume is supplied for duplicate assay if needed.

A practice test and positive control may be purchased in addition to this test.

Analyte/CAS#
OchratoxinA303-47-9
AflatoxinB11162-65-8
AflatoxinB27220-81-7
AflatoxinG11165-39-5
AflatoxinG27241-98-7

Foreign Materials in Hemp
(500mg in hemp)

A 5 sample panel for semi-quantitative determination of foreign materials in hemp matrix. At least 2 of 5 samples will contain foreign materials at levels above 5% by mass. Each vial will contain 500mg of materials for analysis. Participants will report results for each sample of the panel.

A practice test and positive control may be purchased in addition to this test.